DIETARY FIBRES AND BREAD : ATTITUDES , BELIEFS AND KNOWLEDGE AMONG YOUNG POPULATION

The link between dietary fibres and prevention of non-communicable diseases is well documented in the existing scientific literature. Despite that, most of the population still do not have enough knowledge about fibres and their presence in certain food. This study intended to make an insight in the knowledge of the Serbian young population about dietary fibres and bioactive compounds through a questionnaire survey. A survey was conducted to a sample of 173 individuals, (37% male and 63% female) randomly selected among students from elementary, high school and university in the Novi Sad municipality. Topics covered by the questionnaire included knowledge of the term dietary fibre, fibre content in different bread types, the relationship between fibres and health and bioactive compounds sources in food. The results for each of the particular topics addressed varied among studied groups, but generally level of knowledge was related to the education level. The highest overall knowledge about dietary fibres and bioactive compounds was expressed by the university students, although elementary and high school students showed a considerable level of knowledge on the subject. Male participants appeared as more familiar with the questions related to bioactive compounds, while females were more informed on questions regarding dietary fibre. This survey revealed that young population in Serbia is well informed about the contribution of dietary fibre and bioactive compounds in promoting health. However, further expansion of existing knowledge will be beneficial in order to improve general health of the entire community.


INTRODUCTION
In the last decades public interest in healthy diet has increased and thus there has been an increasing demand for healthy foods.An unhealthy diet represents one of the main risk factors for food allergies (Vuksanović et al., 2016) and chronic diseases development (WHO, 2013).Dietrelated diseases affect people across all age groups, social classes and national borders.In addition, young population is the most vulnerable considering important lifestyle changes which cause negative changes in diet.The average student diet is often low in nutrients such as dietary fibres, vitamins, bioactive compounds, low in fruit and vegetable intake, high in sugar, saturated and trans-fat, and sodium intake.Fibre-rich foods include many fruits and vegetables, beans, whole grains, and nuts.Ingestion of fibres from different sources is often coupled with the ingestion of other bioactive compounds that would act synergistically in the human body and contribute to the general wellbeing (Macagnan et al., 2016).Children (9-13 years) should eat 26-31 g of total dietary fibres (TDF) daily, adolescents (14-18 years) 26-38 g of TDF daily, and adults (19-30 years) 25-38 g of TDF daily (Slavin and Jacobs, 2010).Despite the recommendations the amount of dietary fibres present in young population diet is low, being influenced by changes in living arrangements, costs and financial resources, availability of convenience, preferences for eating outhome, presence of refined and processed types of food on the market, and lack of information on this subject (Tarcea et al., 2016;Bagordo et al., 2013).An adequate dietary fibre intake is associated with a range of important health benefits.The physico-chemical characteristics of dietary fibre such as their solubility and chemical structure constitute the starting point for their physiological effects in human body.A significant number of scientific studies confirm the important role of dietary fibres in the prevention of non-communicable chronic diseases such as obesity, colon, rectal and breast cancers, coronary heart diseases and improved glycemic control among diabetics (Sardinha et al., 2014;Kendall et al., 2010).The role of soluble dietary fibre in the digestive tract is reflected in their ability to associate with bile salts and cholesterol in the small intestine, which decreases their absorption.In the large intestine, soluble dietary fibres promote the formation of short-chain fatty acids, which reduces cholesterol synthesis (dos Santos et al., 2016).The main role of insoluble fibres is to facilitate intestinal transit, and thus reduce the colon exposure to the carcinogens and thereby decrease the probability of cancer occurrence (Elleuch et al., 2011).
Bread is a staple food eaten all over the world and as an integral part of the human diet provides carbohydrates including dietary fibres, protein, minerals, vitamins and many other bioactive compounds.Dietary fibres are concentrated in the kernel bran and germ and therefore wholegrain breads have higher fibre content when compared to white bread.Moreover, it is established that high intake of whole grains and cereal fibre showed significant and consistent protective effects on diseases such as type 2 diabetes, cardiovascular disease and colorectal cancer (Huang et al., 2015).Recently, interest shown by consumers regarding dietary fibre has found the response from the food industry through the development of diverse fibre enriched foods (Guiné et al., 2016).
A number of health complications are starting to increase within the young population implying that increase in fibre intake could be beneficial.However, enrichment of regular diet with fibre rich food still remains a public health challenge.There is no representative data available describing the perceptions of young people about dietary fibres or their levels of knowledge in Serbia.Considering this background, the objective of the present study was to estimate the young population knowledge about dietary fibres and bioactive compounds and their presence in different bread types.

Study population
The present study involved 173 students, including 64 males (37%) and 109 females (63%), aged 14-26 years.Participants were selected by random sampling method among students from elementary schools (31), high schools (45) and university (97) in the Novi Sad municipality.The majority of the surveyed university students attended Faculty of Sciences, Faculty of Technology and Faculty of Technical Sciences.The participation in the survey was voluntary, and the questionnaire was applied by direct interview only after verbal consent was obtained.Prior to filling in the questionnaire, participants were informed of the study's aims as well as that responses would be used only for research purposes and the given information would be treated as confidential.

Survey characteristics
The self-designed questionnaire included two major sections.The first section co-vered demographic data including gender and age along with social and cultural data focused on education level (elementary school, high school or university) and degree course.The second section was focused on testing the attitudes and knowledge towards dietary fibres and bioactive compounds among examined population.In this section, participants completed the questionnaire by marking or ranking the offered responses.
Ethical approval for conducting a survey was provided by the Faculty of Technology Ethical Committee.

Data analysis
The answers provided by the survey have been collected in a database using Microsoft Excel 2010.Results were processed using the Statistica 13.2 software (Stat-Soft, Inc., USA.) and expressed in percentages (%) on the total number of participants based on enrolled school and gender.

RESULTS AND DISCUSSION
In Table 1 are presented the characterristics of studied young population regarding education level and gender.The majority of the study population consisted of university students (56%).Considering gender, female participants were more numerous (63%).The obtained results regarding participants' knowledge about the dietary fibre term and their content in different bread types are graphically illustrated in Figure 1.
As regard to the dietary fibre term, high school students showed lack of knowledge since 31.58%were not familiar with this term (Figure 1a).University students prove to be the most informed in the view of dietary fibre term (Figure 1a) which was expected due to the higher education level.Encouraging observation is that 38% of elementary school students heard or read about dietary fibre implying that in the recent years more information of the corresponding subject was introduced to them.Furthermore, the results showed that females have better knowledge of the dietary fibre term compared to male participants (Figure 1a) since the difference was 7% in favour of females.In average 24.33%±9.24participants regarding education level and 26.33%±5.8participants regarding gender showed knowledge of dietary fibre term.These results are consistent with the previous study of Ljubicic et al. (2017) conducted in Croatia.Still, 30-37% of all participants, regardless of education level and gender, are not sure what dietary fibre term means indicating that further attention should be referred to the young population education on this subject.When the question addresses the possible relationships between knowledge about dietary fibre and their presence in various bread types, more than 61% of all participants ranked whole wheat bread as bread with the highest dietary fibre content (Figure 1b).This result indicates either young population awareness that dietary fibres are predominantly located in the bran or increasing influence of market trends.Half white bread is made from partially refined flour (contains bran parts) and thus has higher dietary fibre content compared to white bread.
Nevertheless, neither one of the surveyed participants marked half white bread as first choice revealing lack of knowledge.Similarly to the previous, university students showed the best knowledge on this question too (74.34%±13.14),while differrences among genders were minimal (79.87%±2.27).When participants were asked to rank breads obtained from different grain flours by dietary fibre content, 47% of high school students put rye bread in first place followed by 41% of university students (Figure 2a).The highest percentage of elementary school students put barley bread on first place while other bread types were ranked almost evenly (Figure 2a).Considering the ranking of other bread types, it could be assumed that elementary school students do not possess enough knowledge which could enable better understanding of dietary fibre presence in different grains.In recent years, spelt was introduced to the population as a grain healthier than common wheat since is found to have higher content of soluble and insoluble dietary fibres (Bonafaccia et al., 2000).Familiarity with this fact may explain why 23-41% of participants gave the advantage to spelt bread over barley bread.According to the Food and Drug Administration (FDA), 3 g of β-glucan originating from barley and oat per day has a positive nutritional effect (US-FDA, 2001).However, unfavourable position given to barley bread in this survey is more understandable, since this type of information is not so divulged among the young population.Considering gender, females seem to be more informed on fibre content in different bread types than males (39.17%±3.01).Regardless of education level, approximately half of the participants primarily associated dietary fibre intake with the decreased risk of digestive system diseases (52.56%±5.94)(Figure 2b).Elementary school and university students' subsequently ranked blood sugar and blood cholesterol lowering as positive outcomes of dietary fibre intake.Nevertheless, the connection between cardiovascular disease prevention and dietary fibre consumption seems to be unknown to the majority of the survey participants since only 15% of high school students ranked this response as their first choice.
Comparing male and female, large differrences between ranked answers were not observed since the first choice in both cases was dietary fibres linkage with digestive system diseases (52.77%±3.92)followed by blood sugar, blood cholesterol and cardiovascular diseases.Considering presented results it could be concluded that surveyed population, in general, is aware of dietary fibre positive influence on mentioned diseases which is in accordance with reported results from similar studies ( On Figure 3 are presented responses obtained for the questions about bioactive compounds sources and participants attitudes towards their application in bread. The responses obtained for the question about the bioactive compounds sources revealed that 46% of elementary and 29% of university students affirmed that garlic is rich in bioactive compounds followed by fruits and vegetables, whole wheat grain and spelt grain (Figure 3a).Unlike them, the first choice of high school students was spelt (37%), followed by fruits and vegetables, whole wheat grain and garlic.Compared to female, high percentage (48%) of male participants marked garlic as first choice implying impact of tradition while only 11% of males agreed that fruits and vegetables are rich in bioactive compounds (Figure 3a).On the other hand, females gave slight advantage to fruits and vegetables and spelt over garlic and whole wheat grain.Still, in average 28.64%±17.82participants regarding education level and 34.95%±18.67participants regarding gender marked garlic as source of bioactive compounds.Given the inconsistency observed in the answers of all participants, one can infer that the inquired are familiar but do not possess the desired knowledge about bioactive compounds and their sources.Regarding the question whether bread should be enriched with bioactive compounds more than 46% of all participants answered affirmatively under the influence of the corresponding statement "if is healthily" (50.94%±4.03),but only 20-31% showed absolute certainty (Figure 3b).Discouraging fact is that 24% of university and 21% of high school students did not have an opinion about this, revealing lack of knowledge while 0-10% showed a negative attitude towards bread enrichment.Participants, regarding gender, in high percentage (46-56%) also agreed with bread enrichment with bioactive compounds considering statement that is beneficial for health while 22-26% replied with certainty (Figure 3b).Nevertheless, male participants seem to be more open-minded in terms of bread enrichment with bioactive compounds when compared to females, since no negative answers were obtained.

CONCLUSIONS
This study allowed comparing education level and gender of young population in relation to the attitudes and knowledge about dietary fibre, their presence in different bread types, relation with disease prevention, and bioactive compound sources.The obtained results showed that the higher education level has great influence on the overall knowledge about dietary fibres and bioactive compounds since university students were the most informed on the subject.Considering gender, females showed a higher level of knowledge regarding questions about dietary fibres, while males were more informed on questions related to bioactive compounds.

Figure 1 .Figure 2 .
Figure 1.(a) Level of knowledge about dietary fibre term (b) level of knowledge about dietary fibre content in different bread types