EFFECTS OF DIETARY SORGHUM AND TRITICALE ON PERFORMANCE, CARCASS TRAITS AND MEAT pH IN BROILER CHICKENS

The study was conducted to evaluate the effects of dietary partial corn replacement by white sorghum (WS) or triticale (T) on growth performance, carcass parameters and pH of meat in broilers. One-day-old unsexed Cobb 500 broilers (n=600) were randomly assigned to 3 groups with 4 replicates per treatment. The broilers were fed with isocaloric and isonitrogenous corn-soybean meal control diets (C), corn-WS-soybean meal diets (WS) or corn-T-soybean meal diets (T) for 35 days. The WS or T partially replaced corn; proportions in diets were 275.4 g/kg or 284.0 g/kg (starter), 307.0 g/kg or 308.2 g/kg (grower) and 332.7 g/kg or 335.0 g/kg (finisher). Results showed that performance (body weight gain, feed intake and feed conversion ratio) or carcass traits (carcass yields, breast, leg, wings, liver and abdominal fat percentage) at 35 d were not affected by the inclusion of WS or T in diets. The pH values of breast (Pectoralis major) and thigh (Biceps femoris) muscle at 30 min and 24 h after slaughter were not influenced by the dietary treatments. In conclusion, partial corn replacement with white sorghum or triticale are suitable options for broiler chicken diet, with no adverse effects on growth performance, carcass characteristics or meat pH, as important quality parameters.


INTRODUCTION
The prices volatility and changes in the availability of corn, as major energy source used in poultry feeding, have increased interest in using other feed ingredients produced in large scale (Ravindran, 2013b).Sorghum (Sorghum bicolor L. Moench) and triticale (genus X Triticosecale) are interesting energy ingredients for poultry and pig's diet due to its similar nutritional composition to corn or wheat (Leeson and Summers, 2005; Barneveld and Cooper, 2002).Furthermore, in the current context of climate change, it is expected that in the near future heat re-sistant crops such as sorghum or triticale are routinely utilized as food and feed resources for humans and animals (Selle et al., 2010;García et al., 2013).Previous study reported that the relatively high amount of antinutritional factors contained in old varieties of sorghum (condensed tannins) or triticale (soluble non-starch polysaccharides, NSP) grain can negatively influence the nutritive value of cereals (García et al., 2013; Selle et al., 2010;Korver et al., 2004).However, the use of new varieties of sorghum (low-tannin) or triticale in broiler diet as partial or total corn substitute have been associated with controversial results.Some studies showed a reduction in weight gain and feed intake and in-creased feed conversion in sorghum-based diets (Jacobs and Parsons, 2013; Robertson and Perez-Maldonado, 2010), or negative effects on performance and the intestinal mucosa (Torres et al., 2013), whereas others indicated similar performance (Fernandes et al., 2013;Rocha et al., 2008;Garcia et al., 2005).Several studies suggested that triticale may be incorporated in broiler diets with no major effect on the nutritive value of the diet or bird performance (Çiftci et al., 2003;Pourreza et al., 2007;Zarghi and Golian, 2009), while other findings (Korver et al., 2004;Santos et al., 2008) shown no effect on carcass parameters and quality of poultry meat.This study was conducted to evaluate the effects of dietary partial corn replacement by white sorghum (WS) or triticale (T) on growth performance, carcass parameters and pH of meat in broilers.

MATERIAL AND METHODS
The experimental protocol was approved by the Animal Care Committee of the National Research-Development Institute for Animal Biology and Nutrition (Balotesti, Romania) and birds were treated in accordance with EU Directive 2010/63/EU (OJEU, 2010).

Broilers, diets and sampling
Six hundred one-day-old unsexed Cobb 500 broilers (42.61±1.15g), housed in environmentally controlled conditions, were randomly assigned to 3 groups and kept in floor pens (2.5 m x 0.8 m) with wood shavings.Each dietary treatment was replicated 4 times with 50 birds per replicate pen.
The birds were vaccinated according to the usual protocol against Newcastle disease and infectious bursal disease.Light schedule during the experimental period was 23 h of light and 1 h of dark.

Statistical analysis
The data obtained were analysed using the GLM procedure (SPSS, 2011).Oneway analysis of variance (ANOVA) with the Tukey's comparison test was used to evaluate statistical significance of differences between dietary treatments.The results are given as means and standard error of the mean (SEM).Differences were considered significant at P≤0.05.

Growth performance
The average body weight gain (BWG), feed intake (FI) and FCR (feed conversion ratio), during respective growth phases and overall period are shown in Table 3.

min, pH 24 h -pH values measured at 30 min and 24 h post mortem; C, control diet; WS, white sorghum diet; T, triticale diet; SEM-standard error of mean
There were no significant differences in BWG, FI and FCR (P>0.05)among the dietary treatments during the experimental period, suggesting that partial corn replacement with sorghum or triticale did not negatively affect broilers performance.Mortality percentage was low and did not differ significantly (P>0.05)among the dietary treatments (data not shown).
The results of our study are consistent with previous reports of others (Garcia et al., 2013;Torres et al., 2013;Stringhini et al., 2009;Rocha et al., 2008;Santos et al., 2006) who did not find any differences in feed intake, weight gain or feed conversion when evaluating the growth performance of broilers fed sorghum as partial corn replacement in diets.Zarghi and Golian, (2009) also, revealed that 50% corn replacement with triticale had no negative effect on broiler growth performance.Mierlita, (2008) suggested that replacing 30% of the corn with triticale did not significantly influence performance and carcass traits, while 60% substitution ratio affected weight gain, feed intake, FCR, carcass parameters and carcass fat quality.Conversely, Baser and Yetisir (2014) showed that partial or total replacement of corn with triticale in diet impaired broilers growth performance and carcass parameters.

Carcass characteristics
Carcass yield and carcass cut-up parts yield of broiler chickens at 35 d (Table 4) were not significantly affected by the dietary treatments (P>0.05).In previous studies related with dietary partial corn replacement with sorghum (Torres et al., 2013;Garcia et al., 2013;Stringhini et al. 2009;Kumar et al. 2005) or triticale (Zarghi and Golian, 2009) no negative effect on carcass yield and/or cut-up parts was observed.

Muscle pH
An important factor in chicken meat quality evaluation is pH.Functional properties of meat dependent on glycolytic reactions which occur during rigor mortis, affecting meat pH directly.Also, the fasting period is related to meat pH and affects the incidence of pale soft exudative (PSE) meat in broiler chickens (Komiyama et al., 2008).As shown in Table 5 the pH values of breast (Pectoralis major) and thigh (Biceps femoris) muscle at 30 min and 24 h after slaughter were not influenced by the treat-ments.Breast and thigh muscle pH was not affected (P>0.05) by the partial corn replacement by white sorghum or triticale in the diet.The pH values obtained were in the normal limits for this parameter (5.7-5.96 for breast and 6.0-6.2 for thigh meat; Fletcher et al., (2000).Similarly, Garcia et al., (2013) reported that partial substitution of corn by sorghum in the diet did not influence breast and thigh meat pH values.

Table 1 .
Chemical composition and amino acid profile of cereals grain used in broiler diets

-essential amino acids 3.502 4.059 5.655Table 2 .
Ingredient and nutrient composition of broiler diets for different growth phases Ingredients

Table 3 .
Effects of dietary treatments on growth performance of broilers 1Means of 50 broilers per replicate; C, control diet; WS, white sorghum diet; T, triticale diet; SEM-standard error of mean; BWG, body weight gain; FI, feed intake; FCR, feed conversion ratio; * Means within rows do not differ significantly (P>0.05)

Table 4 .
Effects of dietary treatments on carcass characteristics of broilers at 35 d

Table 5 .
Effects of dietary treatments on muscle pH of broilers at 35 d *Means within rows do not differ significantly (P>0.05);pH 30